B-POL® High-Fidelity Polymerase
In-vitro nucleic-acid amplification systems are among the most widely used tools in biotechnology, with applications ranging from research and development to molecular diagnostics. Nucleic-acid amplification systems developed with B-POL® are engineered to deliver high yield and sensitivity in every reaction. Moreover, the use of product-specific quality controls ensures excellence and reliability.
High Yield and Sensitivity in Every
Reaction
B-POL® is a high-fidelity polymerase produced through recombinant-DNA technology, designed via chimeric protein engineering. This enzyme offers processivity and fidelity equal to or greater than leading international brands.
APPLICATIONS
For many years, biotechnology applications in academia and industry have relied on imported supplies.
Long lead times and price volatility can jeopardize the projects and services that depend on these products.
FEATURES & BENEFITS
A high-fidelity polymerase produced with state-of-the-art recombinant technology and bioprocess engineering. It is ideal for PCR amplification of DNA fragments in applications that demand exceptional copy accuracy.
The enzyme has proofreading activity (3′-5′ exonuclease) capable of correcting errors during DNA replication.
Each B-POL® kit contains:
- 10 × reaction buffer
- MilliQ-grade water
- 50 mM ionic solution
- B-POL® high-fidelity enzyme
CONTENTS
What’s included in the KIT
Enzyme 100 Units (100 reactions)
STOCK concentration: 1 Unit/μL in storage buffer (50 % glycerol, 100 mM Tris-HCl pH 8.0, 0.2 mM EDTA, 2 mM DTT, 0.2 % NP-40, 0.2 % Tween 20).
10× Reaction Buffer
STOCK concentration: 10×, dilute to 1× in the final reaction.
Composition at 1×: 20 mM Tris-HCl, 10 mM KCl, 10 mM (NH₄)₂SO₄, 2 mM MgSO₄, 0.1 % Triton X-100.
50 mM Ionic Solution (MgCl₂)
STOCK concentration: 50 mM. Recommended final concentration: 1 mM. Depending on amplicon difficulty, MgCl₂ can be adjusted between 1 mM and 6 mM.
Water
Sterile milli-Q grade water.
STORAGE
- B-POL® is a chemically prepared commercial enzyme designed to be stored under ideal conditions at −20 °C.
- Frost buildup around the product must be avoided, because excessive moisture shortens shelf life.
- Store the enzyme horizontally inside its package; never flip it upright. The internal tubes are not completely filled, so standing them vertically can leave enzyme coating the tube walls and compromise chemical stability.
- Verify that the freezer temperature remains at −20 °C by logging the reading at least once a week.
- A B-POL® kit contains polymerase, buffer, ionic solution (Mg²⁺), and water. The polymerase and buffer are the most temperature-sensitive components; in an emergency, protect these first. The remaining components can withstand 4 °C (refrigerator temperature) for a couple of days without significant loss of quality.
TRANSPORT
- B-POL® must be kept at −20 °C, so it must be shipped either with cold packs that reach −20 °C (note: gel packs come in different temperature ranges) or on dry ice (solid CO₂).
Polystyrene foam coolers (dry-ice chests) are recommended for shipping; if logistics allow, any insulated container that maintains a cold interior is acceptable.
It is critical to keep the enzyme at −20 °C even during short transfers.
Place the enzyme (kit box) so that it travels horizontally to prevent the reagent from spreading along the tube walls, which could shorten shelf life; make sure the kit is firmly secured inside the cooler.
Avoid excessive moisture around the package.
Formats
| Format | B-Pol® Reactions Estimated Yield 1 Unit = 1 Reaction |
K-100 | 1 Kit 100 U | 100 |
10K-100 | 1 Kit 1000 U | 1000 |
BK100-100 | BULK 100 Kit 100 U | 10,000 |
BK1000-100 | BULK 1000 Kit 100 U | 100,000 |